F4/80 Recombinant Rabbit mAb (SDT-313-49),specification,price,image-Bio-Equip in China

Product Detail
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Product Specification

Host	Rabbit
Antigen	F4/80
Synonyms	Adhesion G protein-coupled receptor E1, EGF-like module receptor 1, EMR1 hormone receptor, Gpf480, Adgre1
Immunogen	Synthetic Peptide
Location	Cell membrane
Accession	Q61549
Clone Number	SDT-313-49
Antibody Type	Recombinant mAb
Application	WB, IHC-P, ICC
Reactivity	Ms, Rt
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000
IHC-P	1:500
ICC	1:100

Background

The F4/80 monoclonal antibody has been used widely as a marker for mouse macrophages [PMID: 16087400]. EMR1-F4/80 is mainly present in the macrophage-containing stroma-vascular fraction. Furthermore, fibroblasts-like cells (adipoblasts), preadipocytes and adipocytes from the murine cell lines, 3T3-F442A and BFC-1, express CD14 and CD68 mRNA and protein as determined by fluorescence-activated cell sorter, but not F4/80 which, as expected, is strongly expressed in the macrophage cell line RAW264.7 [PMID: 16213494].