hnRNP I/PTB belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. Heterogeneous nuclear ribonucleoprotein I encoded by hnRNP I/PTB has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. Heterogeneous nuclear ribonucleoprotein I is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described.

Human Heterogeneous nuclear ribonucleoprotein I (hnRNP I/PTB) ELISA Kit employs a two-site sandwich ELISA to quantitate PTBP1 in samples. An antibody specific for PTBP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTBP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTBP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTBP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Heterogeneous nuclear ribonucleoprotein I (hnRNP I/PTB) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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