Parathyroid hormone receptor 1 functions as a receptor for parathyroid hormone (PTH) and for parathyroid hormone-like hormone (PTHLH).This "classical" PTH receptor is expressed in high levels in bone and kidney and regulates calcium ion homeostasis through activation of adenylate cyclase and phospholipase C. In bone, it is expressed on the surface of osteoblasts. When the receptor is activated, these cells in turn stimulate osteoclasts to ultimately increase the resorption rate.It is a member of the secretin family of G protein-coupled receptors. The activity of this receptor is mediated by Gs G proteins which activate adenylyl cyclase. Besides, they also activate phosphatidylinositol-calcium second messenger system.Expressed in most tissues. Most abundant in kidney, bone and liver.

Human Parathyroid Pormone 1 Receptor (PTH1R) ELISA Kit employs a two-site sandwich ELISA to quantitate PTH1R in samples. An antibody specific for PTH1R has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTH1R present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTH1R is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTH1R bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Parathyroid Pormone 1 Receptor (PTH1R) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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