Tyrosine-protein kinase 6 is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation.The deduced 451-amino acid polypeptide sequence was composed of 3 domains: an SH3 domain, an SH2 domain, and a catalytic domain. The sequence of BRK, unlike that of SRC, does not include an N-terminal myristoylation domain.

Human Protein-tyrosine kinase 6 (PTK6) ELISA Kit employs a two-site sandwich ELISA to quantitate PTK6 in samples. An antibody specific for PTK6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTK6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTK6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTK6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Protein-tyrosine kinase 6 (PTK6) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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