The deduced human ADPRH protein has 357 amino acids and an estimated molecular mass of 39.5 kD. Human ADPRH shows 83% amino acid identity to rat Adprh and 82% identity to mouse Adprh. The authors found that the mouse and rat Adprh sequences contain 5 conserved cysteine residues; the human sequence contains only 4 cysteines, with cys108 in rat corresponding to ser103 in human.Expression of recombinant wildtype and mutant rat and human ADPRH proteins in E. coli and assessment of their DTT requirements for hydrolase activity demonstrated that cys108 is responsible for the DTT dependence of rat Adprh. Northern blot analysis detected a 4.1-kb ADPRH transcript in various human tissues, including fetal and adult brain.

Human [Protein ADP-ribosylarginine] hydrolase (ADPRH) ELISA Kit employs a two-site sandwich ELISA to quantitate ADPRH in samples. An antibody specific for ADPRH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADPRH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADPRH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADPRH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human [Protein ADP-ribosylarginine] hydrolase (ADPRH) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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