Ludwig and Seuwen (2002) cloned full-length ADCY5. EST database analysis revealed 2 alternative polyadenylation sites. The deduced protein contains 1,261 amino acids. Semiquantitative RT-PCR detected high expression of ADCY5 in heart and testis, moderate expression in brain, prostate, ovary, small intestine, and colon, and low expression in lung and liver.Gaudin et al. (1994) mapped the ADCY5 gene to chromosome 3. Using isotopic in situ hybridization, Haber et al. (1994) mapped the ADCY5 gene to 3q13.2-q21. By fluorescence in situ hybridization, Edelhoff et al. (1995) mapped the ADCY5 gene to human chromosome 3q13 and to mouse chromosome 16 in the B5 region. In both species, the gene maps close to GAP43 .

Human Adenylate cyclase type 5 (ADCY5) ELISA Kit employs a two-site sandwich ELISA to quantitate ADCY5 in samples. An antibody specific for ADCY5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADCY5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADCY5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADCY5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Adenylate cyclase type 5 (ADCY5) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.