The predicted 860-amino acid ADAMTS6 protein has a domain structure characteristic of ADAMTSs. Beginning at the N terminus, it has a preproregion containing a potential furin cleavage site and a putative cysteine-switch, a catalytic domain containing a typical reprolysin-type zinc-binding signature and a 'met turn,' a disintegrin-like domain, a conserved thrombospondin module, a cysteine-rich domain, a spacer domain, and a second, less conserved thrombospondin module. The mature ADAMTS6 protein has 2 potential N-linked glycosylation sites. Northern blot analysis of several human tissues detected an approximately 8.5-kb ADAMTS6 transcript that was expressed at low levels in placenta and at barely detectable levels in a number of other tissues. Adamts6 was not detectably expressed during mouse embryo development.

Human A disintegrin and metalloproteinase with thrombospondin motifs 6 (ADAMTS6) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAMTS6 in samples. An antibody specific for ADAMTS6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAMTS6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTS6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTS6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human A disintegrin and metalloproteinase with thrombospondin motifs 6 (ADAMTS6) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-a-disintegrin-and-metalloproteinase-with-thrombospondin-motifs-6-adamts6-elisa-kit-kte60922/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.