ABCB5 [ATP-Binding Cassette Subfamily B (MDR/TAP) Member 5] is a human ABC transporter and P-glycoprotein family member principally expressed in physiological skin and human malignant melanoma.ABCB5-alpha and -beta diverge after exon 6, with ABCB5-alpha including a seventh exon that encodes its 3-prime UTR, and ABCB5-beta including 14 more exons. The 131-amino acid ABCB5-alpha protein has a calculated molecular mass of 15 kD. It has an ABC signature motif and a Walker B consensus sequence, but no Walker A consensus sequence. In contrast, ABCB5-beta has an N-terminal ABC signature motif and Walker B motif, followed by 6 transmembrane domains and C-terminal Walker A, ABC signature, and Walker B motifs.

Human ATP-binding cassette sub-family B member 5 (ABCB5) ELISA Kit employs a two-site sandwich ELISA to quantitate ABCB5 in samples. An antibody specific for ABCB5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCB5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCB5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCB5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human ATP-binding cassette sub-family B member 5 (ABCB5) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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