Cbfa1/Runx2 is a key transcription factor associated with osteoblast differentiation.This protein is a member of the RUNX family of transcription factors and has a Runt DNA-binding domain. It is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Transcript variants of the gene that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing.The RUNX2 transcription factor is the master regulator of osteoblast differentiation is required for chondrocyte hypertrophy .

Human Runt-related transcription factor 2 (RUNX2) ELISA Kit employs a two-site sandwich ELISA to quantitate RUNX2 in samples. An antibody specific for RUNX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRUNX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RUNX2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RUNX2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Runt-related transcription factor 2 (RUNX2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.