Estradiol is an estrane (C18) steroid. It is also known as 17β-estradiol (to distinguish it from 17α-estradiol) or as estra-1,3,5(10)-triene-3,17β-diol. It has two hydroxyl groups, one at the C3 position and the other at the 17β position, as well as three double bonds in the A ring. Due to its two hydroxyl groups, estradiol is often abbreviated as E2. Estradiol is the primary female sex hormone.

Bovine 17β-estradiol (17β-E2) ELISA Kit employs a two-site sandwich ELISA to quantitate 17β-E2 in samples. An antibody specific for 17β-E2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any17β-E2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 17β-E2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 17β-E2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Bovine 17β-estradiol (17β-E2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.