Bax (Bcl-2 associated x protein) is an apoptosis regulating protein of the Bcl-2 family. The ratio of Bax to Bcl-2 determines survival or death following an apoptotic stimulus. Bax is implicated in many cancers, uterine leiomyomas, and brain dysmorphology. Bax is a 21 kDa protein that accelerates apoptosis by binding to, and antagonizing the death repressor activity of Bcl-2 in vivo.Bax normally resides in the cytoplasm, but following an apoptotic stimuli, Bax undergoes a conformational change and translocates to mitochondrial membranes, where it inserts and mediates the release of cytochrome c from the intermembrane space into the cytosol, in this manner activating caspase-3 and perpetuating the apoptotic cascade.

Bovine Apoptosis regulator BAX (BAX) ELISA Kit employs a two-site sandwich ELISA to quantitate BAX in samples. An antibody specific for BAX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBAX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BAX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BAX bound in the initial step. The color development is stopped and the intensity of the color is measured.

Bovine Apoptosis regulator BAX (BAX) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.