By searching an EST database for sequences containing the alpha-crystallin domain characteristic of small heat-shock proteins, followed by PCR of genomic DNA, Kappe et al. (2001) cloned HSPB9. The deduced protein contains 159 amino acids. Northern blot analysis of several human tissues detected a 0.8-kb transcript only in testis. In situ hybridization of adult mouse testis showed expression of Hspb9 in spermatogenic cells from late pachytene spermatocyte stage until the elongate spermatid stage.Kappe et al. (2001) determined that the HSPB9 gene is intronless. By genomic sequence analysis, Kappe et al. (2001) mapped the HSPB9 gene to chromosome 17.

Bovine Heat shock protein beta-9 (HSPB9) ELISA Kit employs a two-site sandwich ELISA to quantitate HSPB9 in samples. An antibody specific for HSPB9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHSPB9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSPB9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSPB9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Bovine Heat shock protein beta-9 (HSPB9) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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