REAGEN^™Aflatoxin M1 ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Aflatoxin M1 in milk,milk powder, starch, vegetable oils, yogurt, buttermilk and soured milk..

The unique features of the kit are:

• No need to extract the sample. The sample can be directly used for the ELISA
• High sensitivity (0.02 ng/g or ppb) and low detection limit in milk(0.05ppb).
• A quick ELISA assay(30 minutes ELISA reaction).
• High reproducibility.

 

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The plate wells are coated with capture antibodies directed against anti-Aflatoxin M1 antibodies. Aflatoxin M1 standards or sample solution, Aflatoxin M1–HRP conjugate and anti-Aflatoxin M1 antibodies are added. Free Aflatoxin M1 and Aflatoxin M1-HRP Conjugate compete for the Aflatoxin M1 antibody binding sites. At the same time, the anti- Aflatoxin M1 antibodies are also bound by the immobilized capture antibodies. Any unbound Aflatoxin M1-HRP Conjugate is then removed in a washing step. The TMB Substrate is added to the wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.

 

 

REAGEN LLC is a high-tech bio-medical enterprise located,We focus on designing and producing all kinds of ELISA kits including:
clinical disease detecting kits
food & feed safety test kits
animal epidemic virus detecting kits
kits for research
microplate readers & washers
other related instruments and equipment