Intended use

Immunoassay for the in vitro quantitative determination of free triiodothyronine in human serum.
 

Summary
Triiodothyronine is one of the thyroid hormones present in serum which regulates metabolism. Determination of this hormone
concentration is important for the diagnostic differentiation of euthyroid, hyperthyroid, and hypothyroid states. The major fraction of total triiodothyronine is bound to the transport proteins (TBG, prealbumin, albumin). Free triiodothyronine (fT3) is the
physiologically active form of the thyroid hormone triiodothyronine (T3). The determination of free T3 has the advantage of being independent of changes in the concentrations and binding properties of the binding proteins; additional determination of a binding parameter (T-uptake, TBG) is therefore unnecessary. (1, 2, 3) In normal thyroid function, as the concentrations of the carrier proteins alter, the total T3 level changes so that the FT3 concentration remains constant. (4) Thus, measurements of FT3
concentrations correlate more reliably with clinical status than total T3 levels. For example, the increase in total T3 levels associated with pregnancy, oral contraceptives and estrogen therapy result in higher total T3 levels while the FT3 concentration
remains basically unchanged. (5) In addition, it has been found that the mean FT3 value has a gradient decreasin from young to older. (6)

 

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