Sample Types Validated:Serum, blood plasma,Saliva, Urine, and other related tissue Liquid.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Please read this insert completely prior to using the product. This kit is used to assay Human Apolipoprotein A1(apo-A1) on the basis of the Biotin double antibody sandwich technology. This Elisa kit takes one-step method with which solutions do not need diluting,because we simplifies the dilute process by our lab techniques. This kit is for research only and is not for use in diagnostic procedurs.

Intended Use

This kit is used to assay the Apolipoprotein A1(apo-A1)in the sample of Human’s serum, blood plasma and other related biological liquid.

Test principle

This kit uses enzyme-linked immune sorbent assay (ELISA) based on the Biotin double antibody sandwich technology to assay the Human Apolipoprotein A1 (apo-A1). Add Apolipoprotein A1(apo-A1)to the wells, which are pre-coated with Apolipoprotein A1(apo-A1)monoclonal antibody and then incubate. After that, add anti apo-A1 antibodies labeled with biotin to unite with streptavidin-HRP, which forms immune complex. Remove unbound enzymes after incubation and washing. Add substrate A and B. Then the solution will turn blue and change into yellow with the effect of acid. The shades of solution and the concentration of Human Apolipoprotein A1 (apo-A1) are positively correlated.

Materials supplied in the Test Kit

1	Standard solution(9.6mg/dl)	0.5ml	7	Chromogen solution A	6ml
2	Standard dilution	3ml	8	Chromogen solution B	6ml
3	Coated ELISA plate	12-well * 8 tubes	9	Stop solution	6ml
4	Streptavidin-HRP	6ml	10	Instruction	1
5	Washing concentrate (30X)	20ml	11	Seal plate membrane	2
6	Anti apo-A1 antibodies labeled with biotin	1ml	12	Hermetic bag	1

Materials required but not supplied

1. 37 ℃ incubator                              2. Standard Enzyme reader

3. Precision pipettes and Disposable pipette tips      4. Distilled water

5. Disposable tubes for sample dilution             6. Absorbent paper

 

Contact  Rebecca Yan 

Skype: span.biotech

Email: rebecca@spanbio.com

Tel:  +86(755)89589611

Fax: +86(755)89580096

Web:

www.spanbiotech.com

Span Biotech Ltd. is a research based company for rapid tests, with strong support from National Key Laboratory of Technology Projects of 10th and 11th Five-Year Plan and Faculty of Life Sciences of HuBei University. SpanBio also housed a R&D team that is developing gene recombination, cell cultivation and protein purification techniques. SpanBio pays strict attention on rapid tests for human being, animal diseases and food safety detection. It provides a number of customized services to professional distributors and partnering affiliates with excellent quality, competitive prices and super service.
Efficiently structured to provide lasting and economic health care solutions with export ready goods, our main products cover the following immunodiagnostic rapid test kits:
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