[TEST PRINCIPLE]
This kit is a solid phase sandwich time-resolved fluorescence immunoassay. The microtiter plate provided in this kit has been pre-coated with the specific antibodies against to AFP. The serum samples are added and the AFP in serum will be captured. When adding the second antibodies to the 96T microtiter plate, the labelled Eu3+ will react with the bound AFP . In this case a multilayer immune complex（Ab-AFP -Ab-Eu3+）will be formed in the microtiter plate. Then the unbound Eu3+ labelled antibodies will be removed by a washing step. Adding the fluorescence enriched solution, the Eu3+ in the immune complex on the surface of the microtiter plate will be dissociated by fluorescence enriched solution and become stable fluorescent complexes. Detect the light intensity with a time-resolved fluorescence immunoassay analyzer and create the reference curve by comparing the light intensity of the calibrators to their corresponding AFP concentrations. For the best results we recommend log/log coordinates to get a reference curve, the AFP concentrations of the unknown samples will be calculated by referring the light intensity to the reference curve.
bio-equip.cn
Wuxi Donglin Sci & Tech Development Co.,Ltd., a manufacturer and supplier of biological reagents for life science, is located in the scenic area beside Taihu Lake. At the inception in 1997, our company focused on providing OEM service of ELISA kits across the world. In 2013, we launched our own brand Dldevelop.
We mainly specialize in manufacturing ELISA kits, distributed to Asia, Oceania, Africa, Europe and America. Headquartered in Wuxi, China, we have an overseas branch in Canada, a domestic branch in Shanghai, China.
Selected raw materials and state-of-the- art technologies help ensure the superior quality of our products with a one-year shelf life, which is six months longer than other manufacturers.
We have more than 6000 ElISA kits available. With the advance of our technology , more kinds of and more specific ELISA kits will be developed and manufactured to meet scientists and researchers’ need.
we strictly obey national and international QC requirements. Our company is a ISO9001:2008 and CE certificated enterprise.
Based on our strong innovative R&D teams, we are always committed to providing global scientists and researchers with the high quality ELISA kits and the best customer experience.
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