Canine Echinococcus Granulosus ELISA Kit,specification,price,image-Bio-Equip in China

Test Principle

Canine Echinococcus Granulosus ELISA Kit is sandwich-antibody enzyme linked immunosorbent assay designed to detect the presence of antigen

to canine Echinococcus Granulosus in the canine stool. A microtitration format has been

configured by coating canine Echinococcus antibody in alternating columns on the plate.

Upon incubation of the test sample in the coated well, antigens specific to canine Echinococcus

Granulosus forms a complex with the coated viral antibodies. After washing away unbound

material from the wells, an anti porcine horseradish peroxidase conjugate is added

that binds to any canine antigens attached in the wells.

In the final step of the assay, unbound conjugate is washed away, and enzyme substrate and a

chromogen, TMB, are added to the wells. Stop the reaction by adding stop solution.

Within 15 minutes READ the absorbance at 450 nm using 630 nm as the reference wavelength

if available.

Enzyme immunoassays for the qualitative of antigens against canine Echinococcus Granulosus

the stool samples.

Materials Supplied

1 Microtiter Plate 1 pieces (96 wells)

2 Negative control 1 tube

3 Positive control 1 tube

4 Enzyme Conjugate 1 bottle

5 Washing buffer 2 bottle

6 Substrate A 1 bottle

7 Substrate B 1 bottle

8 Stop solution 1 bottle

9 Sample diluent 2 bottle