Test Principle

Canine Echinococcus（Ech）Coproantigens ELISA Kit is sandwich-antibody enzyme linked immunosorbent assay designed to detect the presence of antigen to canine Echinococcus Coproantigens in the canine stool. A microtitration format has been configured by coating canine Echinococcus antibody in alternating columns on the plate. Upon incubation of the test sample in the coated well, antigens specific to canine Echinococcus Coproantigens forms a complex with the coated viral antibodies. After washing away unbound material from the wells, an anti-porcine horseradish peroxidase conjugate is added that binds to any canine antigens attached in the wells. In the final step of the assay, unbound conjugate is washed away, and enzyme substrate and a chromogen, TMB, are added to the wells. Stop the reaction by adding stop solution. Within 15 minutes READ the absorbance at 450 nm using 630 nm as the reference wavelength if available.

 

Enzyme immunoassays for the qualitative of antigens against canine Echinococcus Coproantigens in the stool samples.

 

Materials Supplied

1  Microtiter Plate           1 pieces (96 wells)

2  Negative control        1 tube

3  Positive control           1 tube

4  Enzyme Conjugate     1 bottle

5  Washing buffer           2 bottle

6  Substrate A                  1 bottle

7  Substrate B                 1 bottle

8  Stop solution             1 bottle

9  Sample diluent           2 bottle

Ecalbio Co.,Ltd specialize in developing reagents of immunodiagnostic technology and biological systems with higher accuracy andreliability for results of the highest quality.

Ecalbio builds up professional platform to develop more than 10,000 products, including antibodies, antigens, biochemicals, ELISA kits, CLIA kits, and laboratory equipments,etc.

Ecalbio laboratories manufacture Antibodies, Proteins, Food safety ELISA kits, Food safety Rapid Tests, Animal Disease ELISA Kits, Animal Disease Rapid Test Kits, Laboratory equipments with incubators and diagnostic system.

Ecalbio has an enthusiastic and creative R&D team, consisting with a group of experienced technicians in lateral flow immunoassay field, and set up long relationship with first-class institutes,universities,academies, to provide excellent service for human healthy and earth.

Ecalbio are committed to saving lives by ensuring individuals worldwide have access to timely and correct diagnosis. Our products are used in over 100 countries worldwide by:
　Government Laboratories
　Food industry Laboratories
　Institutes
　Veterinary Clinics
　Pharmaceutical Industry.

Ecalbio welcomes potential partners and distributors to explorebusiness relationships with all customer in the worldwide, focused on scie-tech, integrity, quality and service for the customers.

ECALBIO CO.,LTD

HZAU, Luoshi road, Wuhan 430060, China
Tel:86-27-87745856
Email:info@ecalbio.com
Website: www.ecalbio.com