Principle
Liquid-phase blocking enzyme linked immunosorbent assay (LPBE) using the neutralizing monoclonal antibody (mAb) sandwich method (M-LPBE) for detection of Foot-and-mouth disease virus (FMDV) type AsiaI antibodies was developed. The antigen-antibody reaction is carried out in a suspension (or liquid medium), and the antigen is blocked by the homologous antibodies, if present, in the test serum for subsequent detection by guinea pig serum.
The antigen molecules which are not completely blocked by the antibodies in the test serum are trapped to the wells of the ELISA plates with the pre-coated typespecific rabbit antibodies.
Antigen and background controls are added containing antigen without serum and blocking buffer respectively as described in plate layout. Subsequently, the bound antigen is traced by sero-type specific guinea pig serum and anti-guinea pigHRPO conjugate and substrate reaction is followed in a standard ELISA procedure.
Storage
At 2-8℃,12 months.
ECALBIO CO.,LTD
Email: info@ecalbio.com Https: //www.ecalbio.com
Tel: +86-27-87745856 Mobi: +86-18963964485
Add: HZAU, Luoshi Road, Wuhan City 430075, China.
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Ecalbio Co.,Ltd specialize in developing reagents of immunodiagnostic technology and biological systems with higher accuracy andreliability for results of the highest quality.
Ecalbio builds up professional platform to develop more than 10,000 products, including antibodies, antigens, biochemicals, ELISA kits, CLIA kits, and laboratory equipments,etc.
Ecalbio laboratories manufacture Antibodies, Proteins, Food safety ELISA kits, Food safety Rapid Tests, Animal Disease ELISA Kits, Animal Disease Rapid Test Kits, Laboratory equipments with incubators and diagnostic system.
Ecalbio has an enthusiastic and creative R&D team, consisting with a group of experienced technicians in lateral flow immunoassay field, and set up long relationship with first-class institutes,universities,academies, to provide excellent service for human healthy and earth.
Ecalbio are committed to saving lives by ensuring individuals worldwide have access to timely and correct diagnosis. Our products are used in over 100 countries worldwide by:
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Ecalbio welcomes potential partners and distributors to explorebusiness relationships with all customer in the worldwide, focused on scie-tech, integrity, quality and service for the customers.
ECALBIO CO.,LTD
HZAU, Luoshi road, Wuhan 430060, China
Tel:86-27-87745856
Email:info@ecalbio.com
Website: www.ecalbio.com
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