This Human IGFBP-3 ELISA kit is to be used for the in vitro quantitative determination of IGFBP-3 concentrations in serum, plasma, cell culture supernatant, tissue fluid.and other biological fluids. This kit is intended for LABORATORY RESEARCH USE ONLY and is not for use in diagnostic or therapeutic procedures.

This Human IGFBP-3 ELISA kit is a ready-to-use 4-hour solid phase immunoassay capable of measuring InhibinBlevels in serum, plasma, cell culture supernatant, tissue fluid and other biological fluids in the range of 0 to 2000 pg/mL. This assay has shown no cross-reactivity with various other cytokines super-family proteins, and is expected to be used effectively for further investigations into the relationship between Human IGFBP-3 and various diseases.

Summary protocol:
1. Add 100µl of each Standards and samples into appropriate wells.
2. Gently mix for 30 seconds. Cover well and incubate at 37°C for 130 minutes.
3. Wash the microwell strips 5X.
4. Add 100µl of 1×Biotin into each well at the same rate and in the same order as the specimens were added. Gently mix for 30 seconds. Cover well and incubate at 37°C for 60 minutes.
5. Wash the plate by following the procedure in step 3.
6. Add 100µl of 1x HRP into each well at the same rate and in the same order as the Biotin was added. Gently mix for 30 seconds. Cover well and incubate at 37°C for 30 minutes.
7. Wash the plate by following the procedure in step 3.
8. Add 100µl of TMB Substrate into each well at the same rate and in the same order as the HRP was added. Gently mix for 10 seconds. Cover well and incubate at 37°C for 15±10 minutes. Protect from light.
9. Add 100µl of Stop Solution into each well at the same rate and in the same order as the TMB substrate solution was added. Gently mix for 30 seconds.
10. Set the microplate reader wavelength at 450nm and measure the optical density (OD) of each well. The plate should be read within 30 minutes after addition of the stop solution.

TYPICAL DATA

Results of a typical standard run of a Human IGFBP-3 ELISA are shown below. Any variation in standard diluent, operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. The following examples are for the purpose of illustration only, and should not be used to calculate unknowns. Each user should obtain their own standard curve.

Transhold, established in 1999, is a privately held corporation located in national high-tech park of Shanghai jiangtong university longwu base. Transhold is the leading resource for up-to-date product information, product reviews, and new technologies for worldwide life reseach scientists in the bio-technological industry. Our mission statement clearly identifies and sets the direction for our company: “ provide research products of gene tests and immunoassay tests with high quality and a very competitive price to meet the growing needs of international professionals.we aim to speed the progress of research by letting people use our technology in a manner that’s substantially more flexible than that of other tools providers.”

In the past 10 years of development, our company has formed an a wide range of products and a entire professnial technical services to help our customers succeed. We have successful developed a strong line of ELISA kit products including human, rat,mouse,pig,Canine,rabbit and Guinea Pig.Transhlod has won a extensive appreciation for our high quailty products of ELISA kit by life scientists and researchers in European countries and our company has a rich experience in export trade . Further,we also supply a series products for real-time PCR .Hot Start Taq DNA is one of our very compatitive produces. It’s would be your best choice if the requirements of PCR experiments is high. Recently ,we foucs on the research and development of PCR PreMix kit . This produce will enableus to be the leader in the field of PCR technology and to have a solid communication with the scientific community. Our empolyees desire to provide a convenient and time-saving way for customers by using our produces.

Through continuing development, Transhold is committed to building a portfolio of quality products. Using only the highest standards, we deliver our products to the market place at highly quality and affordable prices.It is our goal to continually research and develop novel tests systems to enhance the quality of life research.