1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Chloramphenicol in the tissue, liver, honey, intestine, shrimp, fish, milk and feed etc. The coupling antigens are pre-coated on the micro-well stripes. The Chloramphenicol in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-CAP antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with Chloramphenicol concentration in the sample. This value is compared to the standard curve and the content of the corresponding Chloramphenicol is subsequently obtained.
 
2. Technical specifications
Sensitivity : 0.025 ppb     
Detect time: Approx. 45 min

Detection limit:
Urine, honey, intestine .......................0.05 ppb
Tissue, liver, milk .......................... ....0.025 ppb
Feed .......................... ...........................0.075 ppb
 
Recovery rate:
Tissue, liver ..........................................80±10%
Honey, urine, intestine,serum ............. 70±10%
Milk,feed ...............................................85±15%
 

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Ecalbio Co.,Ltd specialize in developing reagents of immunodiagnostic technology and biological systems with higher accuracy andreliability for results of the highest quality.

Ecalbio builds up professional platform to develop more than 10,000 products, including antibodies, antigens, biochemicals, ELISA kits, CLIA kits, and laboratory equipments,etc.

Ecalbio laboratories manufacture Antibodies, Proteins, Food safety ELISA kits, Food safety Rapid Tests, Animal Disease ELISA Kits, Animal Disease Rapid Test Kits, Laboratory equipments with incubators and diagnostic system.

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ECALBIO CO.,LTD

HZAU, Luoshi road, Wuhan 430060, China
Tel:86-27-87745856
Email:info@ecalbio.com
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