Kanamycin (KM) ELISA Kit,specification,price,image-Bio-Equip in China

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1. Principle

Kanamycin (KM) ELISA Kit is a competitive enzyme immunoassay for the quantitative analysis of Kanamycin in honey, tissue, milk.

The coupling antigens are coated on the micro-well stripes. The Kanamycinin the sample and the coupling antigens coated on the micro-well stripes compete for the anti-KM antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration.

The optical density (OD) value of the sample has a negative correlation with the Kanamycinin it. The measurement is made photometrically at 450 nm. The absorption is inversely proportional to the Kanamycin concentration in the sample.

2. Technical specification:

Sensitivity: 0.025ppb Detect time: Approx.75min

Detect limit:

Chicken, duck, pork, shrimp, fish ........ 2 ppb

Honey ................................................... 2ppb

Milk ......................................... 2 ppb

Cross-reaction rate:

Kanamycin............................................ 100%

Streptomycin ......................................< 1%

Dihydrostreptomycin..........................< 1%

Recovery rate:

85 ± 10%

96 tests/kit