β-Lactams ELISA Test Kit,specification,price,image-Bio-Equip in China

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1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of β-Lactams in the tissue, liver, honey, intestine, shrimp, fish, etc.The coupling antigens are pre-coated on the micro-well stripes. The β-Lactams in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-β-Lactams antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with β-Lactams concentration in the sample. This value is compared to the standard curve and the content of the corresponding β-Lactams is subsequently obtained.

2. Technical specifications

Sensitivity : 0.1 ppb Detect time: Approx. 75 min

Detection limit:

Tissue, liver, honey, shrimp, fish, milk ........... 2 ppb

Recovery rate:

Tissue, liver, honey, shrimp, fish, milk....... 85 ±10%

Cross-reaction rate:

Penicillin ......................100%

Ampicillin...................... 90 %

Amoxicillin..................... 80 %

Nafcillin……………… 110%

Amoxicillin …………… 80%

Piperacillin……………. 80%

Azlocillin...................... 90%

Cloxacillin.....................75%

Dicloxacillin...................50%

Oxacillin ....................... 50%

Metampicillin................50%

Carbenicillin..................10%

Ticarcillin..................... 10%

Cephalosporins...........<0.01