【INTENDED USE】
Enzyme immunoassays for the qualitative of IgG antibodies against PRRSV in pig serum or plasma
【Principles】
The PRRS 2XR Test Kit is an enzyme immunoassay designed to detect the presence of antibody to PRRSV in swine serum. A microtitration format has been configured by coating PRRSV antigen in alternating columns on the plate. Upon incubation of the test sample in the coated well, antibody specific to PRRSV forms a complex with the coated viral antigens. After washing away unbound material from the wells, an anti-porcine: horseradish peroxidase conjugate is added that binds to any porcine IgG antibody attached in the wells. In the final step of the assay, unbound conjugate is washed away, and enzyme substrate and a chromogen, 3,3’,5,5’ tetramethylbenzidine (TMB), are added to the wells. Stop the reaction by adding stop solution. Within 15 minutes READ the absorbance at 450nm using 630nm as the reference wavelength if available.
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Ecalbio Co.,Ltd specialize in developing reagents of immunodiagnostic technology and biological systems with higher accuracy andreliability for results of the highest quality.
Ecalbio builds up professional platform to develop more than 10,000 products, including antibodies, antigens, biochemicals, ELISA kits, CLIA kits, and laboratory equipments,etc.
Ecalbio laboratories manufacture Antibodies, Proteins, Food safety ELISA kits, Food safety Rapid Tests, Animal Disease ELISA Kits, Animal Disease Rapid Test Kits, Laboratory equipments with incubators and diagnostic system.
Ecalbio has an enthusiastic and creative R&D team, consisting with a group of experienced technicians in lateral flow immunoassay field, and set up long relationship with first-class institutes,universities,academies, to provide excellent service for human healthy and earth.
Ecalbio are committed to saving lives by ensuring individuals worldwide have access to timely and correct diagnosis. Our products are used in over 100 countries worldwide by:
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