Mouse IgG is the most abundant antibody isotype in humoral immunity, composed of two γ-heavy chains (γ-chains) and two light chains (κ or λ) linked by disulfide bonds to form a canonical Y-shaped monomeric structure. The γ-heavy chain contains three constant domains (CH1–CH3), with the Fc region (CH2–CH3) mediating binding to Fcγ receptors (FcγRI/II/III) and complement protein C1q. Unlike IgE, IgG lacks the Cε1 domain but features a hinge region that confers molecular flexibility, enhancing antigen-binding diversity. Glycosylation modifications (e.g., N-linked oligosaccharides in the Fc region) regulate IgG effector functions, such as antibody-dependent cellular cytotoxicity (ADCC). Applications of the Mouse IgG ELISA Kit: This kit is designed for quantitative detection of total IgG in mouse serum, plasma, or cell culture supernatants. Advantages of this OneStep ELISA Kit Design: Simplified Workflow: Combines sample incubation and detection in a single step, eliminating intermediate washing and reducing hands-on time. High Efficiency: Achieves results within 45 minutes, ideal for high-throughput studies or urgent diagnostics. Enhanced Precision: Minimizes procedural variability and cross-contamination risks through optimized reagent formulation. Broad Compatibility: Validated for diverse biological matrices, ensuring flexibility in experimental design. This kit’s streamlined protocol and robust performance make it an indispensable tool for researchers and clinicians seeking reliable IgG quantification in time-sensitive or resource-limited settings.