Specimen Requirements

1. Specimens should be extracted as soon as possible after collection. Extraction procedures should be performed according to relevant literature, and experiments should be performed as soon as possible after extraction. If experiments cannot be performed immediately, specimens can be stored at -20°C, but repeated freezing and thawing should be avoided.

2. Samples containing NaN3 cannot be tested because NaN3 inhibits horseradish peroxidase (HRP) activity.

Procedure

1. Dilution of Standard: This kit provides one standard sample at full strength. Users can dilute the sample in a small test tube according to the following chart. 16ng/ml

No. 5 standard

150μl of the original standard was added to 150μl of the standard diluent

8ng/ml

Standard No. 4

150μl of Standard No. 5 was added to 150μl of Standard Diluent

4ng/ml

Standard No. 3

150μl of Standard No. 4 was added to 150μl of Standard Diluent

Standard No. 2

150μl of Standard No. 3 was added to 150μl of Standard Diluent

1ng/ml

Standard No. 1

Cat

Component name

96T

Serial number

Component name

96T

1

30 times concentrated washing liquid

20ml×1 bottle

6

Developer B solution

6ml×1/bottle

2

Enzyme label reagent

6ml×1 bottle

7

Enzyme-labeled coated plate

12 wells×8 strips

8

Standard product (32ng/ml)

0.5ml×1 bottle

4

Sample diluent

6ml×1 bottle

9

Standard diluent

1.5ml×1 bottle

5

Developer A solution

6ml×1 bottle

10

Sealing film

2 sheets