Total Sugar Content Detection Kit (Micromethod),specification,price,image-Bio-Equip in China

Product Detail
Company Profile

Product Specification

Operating Instructions

Plant tissue, animal tissue, serum (plasma)

Theory

Carbohydrate is one of the important components of animals and plants, and is also the main raw material and storage material for metabolism. "Total sugars mainly refer to glucose, fructose, lactose, which are reduced, and sucrose, maltose, which can be hydrolyzed to reduced monosaccharides under the measured conditions, and starch, which may be partially hydrolyzed." The total sugar content detection kit (micromethod) can detect the total sugar content in liquid samples such as animal and plant tissues and serum (pulp). Its principle is to hydrolyse the total sugar into reducing sugar, which reduces DNS to amino compounds after co-heating with DNS reagent under alkaline conditions. In a certain concentration range, the reducing sugar content is linear with the absorbance at 540 nm. According to the standard curve, the total sugar content in the sample can be calculated.

Synonym

Micro Total Carbohydrate Assay Kit

Description

Sensitivity: 0.1 mg/mL

Composition

Name	Size（96T）	Storage
Hydrochloric Acid	100mL	4℃
Sodium Hydroxide	100mL	4℃
DNS Reagent	4mL	4℃, keep in dark place
Standard?	1	4℃

Background

Total sugars mainly refer to glucose, fructose, lactose, which are reduced, and sucrose, maltose, which can be hydrolyzed to reduced monosaccharides under the measured conditions, and starch, which may be partially hydrolyzed. Carbohydrate is one of the important components of animals and plants, and is also the main raw material and storage material for metabolism.

Storage Temp.

2-8 ° C, stored in the dark, valid for 12 months.

Test Range

0.1-2 mg/mL

Applications

Plant tissue, animal tissue, serum (plasma)

Assay Procedure (Click to expand)

Self-supplied consumables:

microplate reader or visible spectrophotometer (can measure the absorbance at 540 nm)

96-well plate or microglass cuvette, adjustable pipet gun and tip

centrifuge, water bath

deionized water

homogenizer (if it is a tissue sample)

reagent preparation

Hydrochloric Acid

ready to use; Before use, equilibrate to room temperature; Store at 4 ° C.

Sodium Hydroxide

ready-to-use type; Before use, balance to room temperature; Store at 4 ° C.

DNS Reagent

ready-to-use; Before use, equilibrate to room temperature; Store away from light at 4 ° C.

Standard

Add 1 mL deionized water to dissolve, prepare 10 mg/mL standard before use; Store at 4 ° C.

standard curve setting: dilute 10mg/mL standard with deionized water to 2, 1.6, 1, 0.8, 0.4, 0.2, 0.1 mg/mL

NO.	10 mg/mL Standard volume (μL)	Deionized water volume (μL)	Concentration (mg/mL)
Std.1	40	160	2
Std.2	32	168	1.6
Std.3	20	180	1
Std.4	16	184	0.8
Std.5	8	192	0.4
Std.6	4	196	0.2
Std.7	2	198	0.1

Sample preparation

Plant or animal tissue samples: Said to take about 0.1 g group, add 1 mL Hydrochloric Acid, 1.5 mL deionized water, homogenate, boiling water bath for 30 min, add 1 mL of Sodium Hydroxide, blending, deionized water constant volume to 10 mL, 8000 g, 25 ℃ centrifuge for 10 min, take on the solution under test.

2, serum (plasma) samples: Take 0.1 mL serum (plasma), add 0.1 mL of Hydrochloric Acid, 0.15 mL deionized water, homogenate, boiling water bath for 30 min, add 0.1 mL Sodium Hydroxide blending, deionized water constant volume to 1 mL, 8000 g, 25 ℃ centrifuge for 10 min, take on the solution under test.

Note

Protein quantification kit abs9232 (BCA assay) is recommended for protein concentration determination.

Experimental procedures

The microplate reader or visible spectrophotometer was preheated for more than 30 min, and the wavelength was adjusted to 540 nm. The visible spectrophotometer was zeroed with deionized water.

2, operation table, in the EP tube, in turn, to join the following reagents:

Reagent	Blank tube（μL）	Standard tube（μL）	Detector tube（μL）
Sample	0	0	30
Different Concentration Std.	0	30	0
Deionized water	30	0	0
DNS Reagent	30	30	30
Mix well, bath in boiling water for 10 min (cover tightly to prevent water loss), remove and cool to room temperature	Mix well, bath in boiling water for 10 min (cover tightly to prevent water loss), remove and cool to room temperature	Mix well, bath in boiling water for 10 min (cover tightly to prevent water loss), remove and cool to room temperature	Mix well, bath in boiling water for 10 min (cover tightly to prevent water loss), remove and cool to room temperature
Deionized water	180	180	180
Blending, take 200 mu to 96 L orifice or trace glass dishes, and determine the hole of absorbance at 540 nm, respectively for A blank, A standard and A determination, determination of calculation Δ A = A - A blank, Δ A = A standard - A blank	Blending, take 200 mu to 96 L orifice or trace glass dishes, and determine the hole of absorbance at 540 nm, respectively for A blank, A standard and A determination, determination of calculation Δ A = A - A blank, Δ A = A standard - A blank	Blending, take 200 mu to 96 L orifice or trace glass dishes, and determine the hole of absorbance at 540 nm, respectively for A blank, A standard and A determination, determination of calculation Δ A = A - A blank, Δ A = A standard - A blank	Blending, take 200 mu to 96 L orifice or trace glass dishes, and determine the hole of absorbance at 540 nm, respectively for A blank, A standard and A determination, determination of calculation Δ A = A - A blank, Δ A = A standard - A blank

The results were calculated as follows:

Note

We provide you with the calculation formula, including the derivation process calculation formula and the concise calculation formula. The two are exactly the same. The concise formula in bold is recommended as the final formula.

1. Drawing of the standard curve

The standard curve was plotted with the concentration of the standard on the Y-axis and the ΔA standard on the X-axis. Will be Δ A measurement into the formula to calculate the y value (mg/mL).

2, sample calculated total sugar content

(1) according to the sample quality is calculated

Total sugar (mg/g)=(y×V sample)÷(W×V sample ÷ total V sample)×n=10×y÷W×n

(2) calculated according to the protein concentration of the sample

Total sugar (mg/mg prot) = (x y V sample) present sample (V (Cpr) * n n = y present Cpr

(3) according to the serum (plasma) volume calculation

Total sugar (mg/mL)=y×V sample ÷(V liquid ×V sample ÷V sample total)×n=10×y×n

V-like

added sample volume, 0.03 mL; The volume V sample total: tissue samples, 10 mL; Cpr: sample protein concentration, mg/mL; W: sample quality, g; V solution: liquid sample volume, 0.1 mL; The volume V sample total: liquid samples, 1 mL; n: dilution factor

General Notes (Click to expand)

Do not mix the components between different batch numbers and different manufacturers; Otherwise, it may lead to abnormal results.

2, when mixing or redissolving the components, avoid air bubbles.

3, frequently change the suction head to avoid cross contamination between the components.

4, before the experiment, ensure that all the components and equipment are at the right temperature.

Before the experiment, it is recommended to select 2-3 samples with large expected differences for pre-experiment. If the absorbance value of the sample is not within the measurement range of the standard curve, it is recommended to dilute or increase the sample size for detection, and multiply the result by the dilution.

6, Hydrochloric Acid and Sodium Hydroxide are corrosive, DNS Reagent has certain toxicity, please take protective measures during operation.

7, this kit for cellulose decomposition degree can not reach 100%.

ANT BIO PTE. LTD. – Empowering Scientific Breakthroughs At ANTBIO, we are committed to advancing life science research through high-quality, reliable reagents and comprehensive solutions. Our specialized sub-brands (Absin, Starter, UA) cover a full spectrum of research needs, from general reagents and kits to antibodies and recombinant proteins. With a focus on innovation, quality, and customer-centricity, we strive to be your trusted partner in unlocking scientific mysteries and driving medical progress. Explore our product portfolio today and elevate your research to new heights.