Detection Principle:

This kit uses double antibody sandwich ELISA technology. The specific anti-human il-12p70capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process; Il-12p70  Bound to solid-phase antibody and detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response and sample   The concentration of IL-12p70 was positively correlated. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Detection Sample Type: cell supernatant, serum, plasma

Loading Volume: 100ul

Kit Components:

Pre coated 96 well plate, standard, IL-12 p70 detection antibody, dilution bufferOne copy of color developing solution (a, b), washing solution, termination solution, sa-hrp, plate sealing film and instructions.

Sensitivity: 0.18pg/ml

Detection Range: 7.81 - 500 pg/ml

Recovery Range: 87-111%

Storage Method: 2-8 ℃

Standard Curve

Background:

Interleukin 12 (IL-12) is naturally produced by dendritic cells, macrophages, neutrophils, and human B lymphoblastoid cell line (NC-37) in response to antigen stimulation. IL-12 consists of 4аHelix is a heterodimeric cytokine encoded by two independent genes, il-12a (p35) and IL-12B (P40). After protein synthesis, bioactive heterodimers (p70) and homodimers P40 can be formed.