Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti mouse IL-12p70 capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. IL-12p70 in the sample binds to the solid-phase antibody. After thorough washing to remove free and unbound components, biotinylated detection antibody was added for incubation. After washing again, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response was positively correlated with the concentration of IL-12p70 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, biotin labeled IL-12p70 detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.57 pg/ml

Detection Range: 7.81-500 pg/ml

Recovery Range: 92-115%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 12 (IL-12) is naturally produced by dendritic cells, macrophages, neutrophils, and human B lymphoblastoid cell line (NC-37) in response to antigen stimulation. IL-12 consists of 4аHelix is a heterodimeric cytokine encoded by two independent genes, il-12a (p35) and IL-12B (P40). After protein synthesis, bioactive heterodimers (p70) and homodimers P40 can be formed. IL-12 is involved in the differentiation of naive T cells into Th1 cells. As a T cell stimulator, it can stimulate the growth and function of T cells. IL-12 has anti angiogenic activity and plays an important role in the activity of natural killer cells and T lymphocytes. Similarly, IL-12 is associated with autoimmunity.