Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti human G-CSF capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The G-CSF in the sample binds to the solid-phase antibody. After thorough washing to remove unbound components, biotinylated detection antibody was added for incubation. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of G-CSF in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, G-CSF detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.19 pg/ml

Detection Range: 15.63-1000 pg/ml

Recovery Range: 94-119%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Granulocyte colony stimulating factor (G-CSF)It is a glycoprotein that stimulates bone marrow to produce granulocytes and stem cells and release them into the blood. Functionally, it is a cytokine and hormone, a colony stimulating factor, produced by endothelial cells, macrophages and many other immune cells. G-CSF stimulates the survival, proliferation, differentiation and function of neutrophil precursors and mature neutrophils. In oncology and hematology, recombinant G-CSF is used to accelerate the recovery of neutropenia caused by chemotherapy in cancer patients. Before leukocyte removal, G-CSF can also be used to increase the number of hematopoietic stem cells in the blood of blood donors for hematopoietic stem cell transplantation.