Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti-human IL-21 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-21 in the sample is combined with the solid-phase antibody and detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-21 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, IL-21 detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 1.03pg/ml

Detection Range: 9.38-600 pg/ml

Recovery Range: 90-116%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 21 (IL-21) has a strong regulatory effect on immune cells, including natural killer cells (NK) that can destroy virus infection or tumor cellsAnd cytotoxic T cells. This cytokine can induce target cell division / proliferation. IL-21 is expressed on human activated cd4+ T cells, but not in most other tissues. In addition, IL-21 was upregulated in helper T cell subsets Th2 and Th17 cells as well as follicular T cells. Moreover, IL-21 is also expressed in NK T cells that regulate the functions of these cells. IL-21 has a pathological role in immune inflammatory diseases. Studies have shown that there is a positive autocrine loop that can amplify and stabilize il-21-driven, T cell-mediated responses. Moreover, it also has anti-tumor activity and plays a role in the process of autoimmunity. In clinic, IL-21 can also be used as a potential antitumor drug.