Detection Principle:

This kit uses double antibody sandwich ELISA technology. The specific anti-human BMP-2 capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. BMP-2 in the sample binds to the solid-phase antibody. After thorough washing to remove unbound components, biotinylated detection antibody was added for incubation. After washing again, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After thorough washing again, TMB chromogenic substrate was added to avoid light for color development. The depth of color response has a positive correlation with the concentration of BMP-2 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value under the condition of 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, BMP-2 detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 1.73pg/ml

Detection Range: 15.63-1000 pg/ml

Recovery Range: 80-121%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Bone morphogenetic protein 2 (BMP-2)Belongs to tgf-β Superfamily proteins. Like other bone morphogenetic proteins, BMP-2 plays an important role in the development of bone and cartilage. BMP-2 participates in Hedgehog pathway and tgf-β The interaction between signaling pathways and cytokines and their receptors is also involved in cardiomyocyte differentiation and epithelial to mesenchymal cell transformation. BMP-2 and BMP-7 are both osteoinductive bone morphogenetic proteins, and experiments have shown that they can effectively induce the differentiation of osteoblasts in many cell types. The use of biconical threaded fusion cage and recombinant human BMP-2 on absorbable collagen sponge can maintain the fusion of intervertebral spine, improve the clinical efficacy, and reduce the pain of patients with degenerative lumbar disc disease after anterior lumbar interbody fusion.