Detection Principle:

This kit uses double antibody sandwich ELISA technology. The specific anti human GDF-15 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The GDF-15 present in the sample is combined with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of GDF-15 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, GDF-15 detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 1.24pg/ml

Detection Range: 15.63-1000 pg/ml

Recovery Range: 79-115%

Rtorage Method: 2-8 ℃

Standard Curve:

Background:

Growth differentiation factor 15 (GDF-15), also known as tgf-pl, MIC-1, PDF, plab and ptgfb, are transforming growth factor beta; A member of the superfamily, which plays a role in regulating inflammatory and apoptotic pathways in damaged tissues and disease processes. Gdf-15mrna mainly exists in the liver, and a small amount also exists in some other tissues. In the injury of organs such as liver, kidney, heart and lung, the expression of GDF-15 in liver is significantly up-regulated.