Detection Principle:

The double antibody sandwich ELISA method was used in this experiment. The anti mouse IL-7 monoclonal antibody is coated on the microplate, the IL-7 in the sample and standard will bind with the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti mouse IL-7 multi antibody was added to combine with IL-7 bound on the microplate to form an immune complex, and the free components were washed away; Add the substrate solution (chromogenic agent), the color of the solution gradually turns blue, add the stop solution, the solution turns yellow and stops changing. The absorbance was measured with a microplate reader.

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Detection Sample Type: cell supernatant, serum, Plasma

Loading Amount: 100ul

Kit Components:

Pre coated 96 well plate, standard, anti mouse IL-7 detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, final stop solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 8.3 pg/ml

Detection Range: 31.2 - 2000 pg/ml

Recovery Range: 85.0-111.9%

Storage Method: 2-8 ℃

Background:

IL-7 (interleukin-7)Is a cytokine that plays an important role in lymphocyte differentiation, proliferation, and survival. IL-7 is produced by stromal epithelial cells of the thymus, bone marrow, and intestine. It is mediated by IL-7 r alpha / CD127 and commonγ The receptor complex composed of chain signalsΓ C is also a subunit of the IL-2, -4, -9, -15, and -21 receptors. IL-7 helps maintain all primitive and memory T cells. Moreover, IL-7 is required for T cell dendritic cell interaction. In mice, IL-7 activation by IL-7 r alpha is essential for the development of both T and B cell lineages, whereas in humans, T cells are not required and B cell development is required.