Detection Principle:

The double antibody sandwich ELISA method was used in this experiment. Anti human mip-1α The monoclonal antibody was coated on the microplate, and mip-1&alpha in samples and standards; It will bind to the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti human mip-1&alpha was added; Polyclonal antibody, and mip-1&alpha bound on the microplate; Combine to form immune complexes, and the free components are washed away; Add the substrate solution (chromogenic agent), the color of the solution gradually turns blue, add the stop solution, the solution turns yellow and stops changing. The absorbance was measured with a microplate reader.

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Detection Sample Type: cell supernatant, serum, Plasma

Loading amount: 100ul

Kit Components:

Precoated 96 well plates, standards, anti human mip-1α One copy of detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 10 pg/ml

Detection Range: 7.81 - 500 pg/ml

Recovery Range: 85.0-111.9%

Storage Method: 2-8 ℃

Standard Curve 

Background:

Mip-1 alpha (macrophage inflammatory protein 1 alpha) is a member of the CC or beta chemokine subfamily, which was initially purified from the conditioned medium of LPS stimulated murine macrophage cell lines. Mip-1& alpha; is highly effective on a variety of cells (including monocytes, T cells, B cells, and eosinophils)Plays a chemotactic role.
Two human mip-1α Genes are generated by duplication / mutation. They encode mip-1α Isoforms CCL3 / ld78a and CCL3L1 / ld78b, which share 94% amino acid sequence homology. Human CCL3 / ld78a is a single copy gene, while human CCL3L1 / ld78b gene copy number varies among populations. Human CCL3L1 / ld78b binds and signals through chemokine receptors CCR1, CCR5. Compared with CCL3 / ld78a, CCL3L1 / ld78b has higher binding affinity for CCR5, which is also a co receptor for HIV-1 entry. The copy number of CCL3L1 is one of several genetic determinants of HIV-1 susceptibility.