Detection Principle:

The double antibody sandwich ELISA method was used in this experiment. The anti human ccl2/mcp-1 capture antibody is coated on the microplate. After incubation, ccl2/mcp-1 in the sample and standard will bind to the antibody fixed on the plate, and the free components will be washed away; Then biotinylated anti human ccl2/mcp-1 detection antibody was added for incubation. After washing to remove unbound substances, streptavidin HRP was added for incubation. After washing, the chromogenic substrate was added to avoid light for color development. The solution color is directly proportional to the bound target protein; Add termination solution; The absorbance was measured with a microplate reader.

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Detection Sample Type: cell supernatant, serum, Plasma

Loading amount: 100ul

Kit Components:

Pre coated 96 well plates, standards, anti human   One copy of ccl2/mcp-1 detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 18.9  Pg/ml

Detection Range: 15.6 - 1000 pg/ml

Recovery Range: 85-104%

Storage Method: 2-8 ℃

Standard Curve 

Background:

CCL2/MCP-1 is a chemokine that binds to the receptor CCR2 and induces chemoattraction of monocytes. It induces the activation of monocytes, NK cells, lymphocytes, and basophils. In addition, CCL2 promotes Th2 polarization of cd4+ T cells, and CCL2 mediated recruitment of monocytes to inflammatory sites exacerbates atherosclerosisSeverity of sclerosis, multiple sclerosis, and allergic asthma. Endogenous proteolytically modified CCL2, including N-terminal pyrrolidone carboxylic acid modified glutamine, downregulated activity but did not bind to the receptor.