Detection Principle:

The double antibody sandwich ELISA method was used in this experiment. Anti human IL-27 monoclonal antibody is coated on the microplate. IL-27 in samples and standards will bind to the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti human IL-27 multi antibody was added to combine with IL-27 bound on the microplate to form an immune complex, and the free components were washed away; Add the substrate solution (chromogenic agent), the color of the solution gradually turns blue, add the stop solution, the solution turns yellow and stops changing. The absorbance was measured with a microplate reader.

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Detection Sample Type: cell supernatant, serum, Plasma

Loading Amount: 100ul

Kit Components:

Pre coated 96 well plate, standard, anti human IL-27 detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 1.8 pg/ml

Detection Range: 156 - 10000 pg/ml

Recovery Range: 82-110%

Storage Method: 2-8 ℃

Standard Curve

Background:

IL-27 is composed of ebi3 (associated with the P40 subunit of IL-12 and IL-23) and p28 (associated with the p35 chain of IL-12)Composed of heterodimeric cytokines. IL-27 is expressed by monocytes, endothelial cells, and dendritic cells through a receptor complex consisting of IL-27 R alpha/wsx-1/tccr and gp130. IL-27 induces IL-12 receptor expression on naive cd4+ T cells, sensitizing them to subsequent IL-12-induced Th1 development. It also acts as an anti-inflammatory cytokine by limiting the activity of T and NKT cells.