Detection Principle:

The double antibody sandwich ELISA method was used in this experiment. Anti human IL-17 monoclonal antibody is coated on the microplate. IL-17 in the sample and standard will bind to the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti human IL-17 multi antibody was added to combine with IL-17 bound on the microplate to form an immune complex, and the free components were washed away; Add the substrate solution (chromogenic agent), the color of the solution gradually turns blue, add the stop solution, the solution turns yellow and stops changing. The absorbance was measured with a microplate reader.

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Detection Sample Type: cell supernatant, serum, Plasma

Loading Amount: 100ul

Kit Components:

Pre coated 96 well plate, standard, anti human IL-17 detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 1.8 pg/ml

Detection Range: 15.6 - 1000 pg/ml

Recovery Range: 82-110%

Storage Method: 2-8 ℃

Standard Curve

Background:

The IL-17 family is composed of at least six pro-inflammatory cytokines, which share a conserved cysteine knot structure, but differentiate at the N terminus. IL-17 family members are glycoproteins secreted as dimers, which can induce the production of local cytokines and recruit granulocytes to the site of inflammation. IL-17 is composed of IL-17 and il-23-induced, mainly in activated cd4+ T cells, unlike Th1 or Th2 cells. IL-17F has the strongest homology with IL-17, but is only induced by IL-23 in activated monocytes. IL-17B binds to the IL-17B receptor, but not to the IL-17 receptor, and has the strongest homology with il-17d, which is expressed by resting cd4+ T cells and cd19+ B cells. Il-17e is mainly produced by Th2 cells and recruits eosinophils to lung tissue. The expression pattern of il-17c is very limited, but it has been detected in adult prostate and fetal kidney banks.