Scoville Heat Units , SHU, can be calculated using Capsaicin ELISA Kit, there is the correlation of Capsaicin Content.
| Pungency Degree |
SHU |
Capsaicin Content |
| 1 |
0-500 |
0-33.3mg/kg |
| 2 |
500-1000 |
33.3mg/kg-66.7mg/kg |
| 3 |
1000-1500 |
66.7mg/kg-100mg/kg |
| 4 |
1500-2500 |
100mg/kg-166.7mg/kg |
| 5 |
2500-5000 |
166.7mg/kg-333.3mg/kg |
| 6 |
5000-15000 |
333.3mg/kg-1000mg/kg |
| 7 |
15000-30000 |
1000mg/kg-2000mg/kg |
| 8 |
30000-50000 |
2000mg/kg-3333.3mg/kg |
| 9 |
50000-100000 |
3333.3mg/kg-6666.7mg/kg |
| 10 |
>100000 |
>6666.7mg/kg |
1. Principle of Capsaicin ELISA Kit
Capsaicin ELISA Kit is a competitive ELISA for the quantitative analysis of Capsaicin in the rapeseed oil, soybean oil, peanut oil, corn oil, blended oil, etc. The coupling antigens are pre-coated on the micro-well stripes. The Capsaicin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Cap antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with Capsaicin concentration in the sample. This value is compared to the standard curve and the content of the corresponding Capsaicin is subsequently obtained.
Enzyme immunoassay (ELISA) is widely used as a screening system, which is more simple, rapid, sensitive and cost-effective compared with HPLC or GC-MS.
2. Technical specifications
Sensitivity : 1ppb Detect time: Approx. 30 min
Detection limit:
Rapeseed oil, soybean oil, peanut oil, corn oil, blended oil ............10ppb
Chili,curry powder & curry paste......................................................100ppb
Recovery rate:
Rapeseed oil, soybean oil, peanut oil, corn oil, blended oil .......... 75±10%
Chili,curry powder & curry paste.....................................................85±10%
Cross-reaction rate:
Capsaicin.............................100 %
Dihydrocapsaicin..................100 %
Nonivamide..........................100 %
ECALBIO CO.,LTD
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ECALBIO CO.,LTD
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Tel:86-27-87745856
Email:info@ecalbio.com
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