The ImmunoCentrix Galactose Screening Assay is an enzymatic colorimetric end-point method for the determination of total D-Galactose in dried blood spot specimens taken from newborn human infants as part of a newborn screening program. 

The ImmunoCentrix Galactose Kit uses trichloroacetic acid (TCA) to extract Galactose from dried blood spot samples. After extraction, the eluted sample is combined with the enzyme reagents Galactose Dehydrogenase and Alkaline Phosphatase . These enzyme reagents catalyzes the NAD-dependent oxidative reaction of Galactose to D-galactono-1,4-lactone + NADH + H+. The NADH produced reacts with a color reagent in which a tetrazolium salt gets reduced producing a distinct color.

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