The Autobio anti-HBs ELISA is designed for the qualitative determination of antibodies to hepatitis B surface antigen (anti-HBs) concentration in human serum or plasma specimens.
Anti-HBs titer can be determined to monitor the prognosis of patients recovering from the hepatitis B viral infection. It also can be used as an indicator of prior exposure to Hepatitis B viruses.
The anti-HBs ELISA is a solid-phase simultaneous immunoassay to detect antibodies against HBsAg. Microwells are coated with hepatitis B surface antigen (HBsAg). A serum specimen is added to the microwells together with horseradish peroxidase (HRP) Labeled HBsAg. After incubation, the complex of antigen-antibody-antigen (HRP-conjugated HBsAg, anti-HBs antibody and HBsAg on the wells) will be formed. Thus, the amount of HRP-HBsAg conjugate bound to the well is proportional to the concentration of anti-HBs in the specimen. The unbound enzyme conjugates will be washed away and then the chromogen and substrate solutions containing hydrogen peroxide is added to the wells. A blue color is developed in proportion to the amount of anti-HBs antibodies the specimens. The enzyme-substrate reaction is terminated with the addition of acid. The absorbance of controls and specimens is read in a microplate reader at the wavelength of 450 nm and 620nm.
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About Group
Autobio is located at Zhengzhou, China and made up of Autobio Co., Ltd and Autobio Diagnostics Co., Ltd., which are all specialized in the field of providing solutions for clinical immunological diagnosis.
With GMP certified facilities and experienced staffs, we have developed high-quality in vitro diagnostic reagents with the competitive price.
In 2007 Autobio Labtec Instruments Co.Ltd. was founded, adding instrumentation to the product portfolio of the Autobio group. A close cooperation with an experienced R&D team in Europe and a highly qualified team in charge of production enables Autobio Labtec instruments to offer state of the art instrumentation to the domestic and international market.
Autobio Co. Ltd.
.. was established in 1998, as a company focusing on the sales and marketing of the range of diagnostic products developed and manufactured within Autobio group.
Autobio Diagnostics Co. Ltd.
... was established in 1999, and has become one of the largest and fastest growing clinical diagnostic companies in China. We are specialized in research & development, production and distribution of clinical diagnostic products which mainly focused on CLIA, ELISA, microbiology and Rapid Test Strips/devices.
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