1.Use purpose
This kit for Feed, fish, shrimp and meat tissue (such as chicken, beef and pork), eggs, honey, milk, serum and urine in the DIOXIN remaining quantitative detection.
 
2 Experimental principle
This kit adopts methods in competition ELISA In microplates coated with antigen conjugated DIOXIN, add DIOXIN standard or samples, Free DIOXIN and pre-coated on strips of DIOXIN conjugated antigen compete against DIOXIN antibody conjugates, With TMB chromogenic substrate,the color is changed from blue to yellow after adding stop solution, enzyme standard instrument 450nm wavelengths in testing, absorb light value and in the sample was DIOXIN content is inversely proportional to the standard curve, through calculation samples was DIOXIN concentrations.
 
3 Materials provided with the kit
3.1 Microelisa stripplate: 1 block (12well×8strips).
3.2 DIOXIN standard: six vials of (1ml/ vial), content is respectively: 0 PPB, 0.1 PPB, 0.3 PPB and 0.9 PPB, 2.7 PPB, 8.1 PPB.
3.3 Anti- DIOXIN antibody conjugate: 1 vial (6ml).
3.4 Chromogen Solution A: 1 vial (6ml).
3.5 Chromogen Solution B: 1 vial (6ml).
3.6 Stop Solution: 1 vial (6ml), 2M sulphuric acid.
3.7 sample dilution: 1 vial (10 x, 6ml), used for sample diluted with.
3.8 wash solution: 1 vial (20 x, 20ml), used for washing board.
3.9 Instruction.
 
4 Need not provide materials
4.1  equipment
4.4.1  wavelength 450nm microplate.
4.1.2  shredder.
4.1.3  LiangTong.
4.1.4  oscillators.
4.1.5  funnel.
4.1.6  Whatman No 1 or equivalent filter paper.
4.1.7  trace remove liquid device.
4.2  reagent
4.2.1 the deionized water or distilled water.
4.2.2 methanol.
 
5 Storage
5.1 kit stored in 2 ~ 8 ℃, Don’t frozen
5.2 Don’t use up the Microelisa stripplate should be sealed drying preserve

Rebecca Yan

Span Biotech Ltd. is a research based company for rapid tests, with strong support from National Key Laboratory of Technology Projects of 10th and 11th Five-Year Plan and Faculty of Life Sciences of HuBei University. SpanBio also housed a R&D team that is developing gene recombination, cell cultivation and protein purification techniques. SpanBio pays strict attention on rapid tests for human being, animal diseases and food safety detection. It provides a number of customized services to professional distributors and partnering affiliates with excellent quality, competitive prices and super service.
Efficiently structured to provide lasting and economic health care solutions with export ready goods, our main products cover the following immunodiagnostic rapid test kits:
Rapid Tests for Human Use :
1) Fertility: Pregnancy HCG, ovulation LH, menopause FSH
2) Infectious diseases: Hepatitis B, HCV, HIV
3) Drug abuse: AMP, BAR, BZO, COC, mAMP, MOP, MTD, OPI, PCP, TCA, THC
4) Tumor markers: CEA, PSA, AFP, FOB
5) STDs(Sexually Transmitted Diseases): Gonorrhea, syphilis, chlamydia
6) New products: Malaria, H. Pylori, Multi-Hepatis B Panel (HBsAg, Anti-HBs, HBeAg, Anti-HBe, Anti-HBc), Blood Glucose Test, Multi-drug of Abuse Panel, Avian (Bird) Flu Rapid Test, etc.
Rapid Tests for Veterinary :
1) Bovine: Cow Pregnancy , Cow Ovulation, Bovine Brucella, Bovine Tuberculosis ,Food and Mouth Disease NSP Ab Test
2) Canine: Canine Distemper, Canine Parvovirus, Canine Coronavirus
3) Feline : Feline Leukemia Virus Ag Rapid Test ,Feline Panleucopenia Virus Ag Test,Feline Immunodeficiency Virus Ab Test
Rapid Tests for Food Safety :
1)For Antibiotics Residue : Nitrofurans (AOZ,AMOZ, AHD, SEM), Streptomycin , Sulfonamides , Chloramphenicol , Quinolone , Beta-Lactam&Tetracycline Combo (2 in 1), β-Lactams & Streptomycin & Chloramphenicol & Tetracyclines Combo (4 in 1), Beta-Lactam, Tetracycline test
2) For Beta-Agonist Residue : Ractopamine, Clenbuterol ,Salbutamol