1. Principle
Crystal Violet ELISA kit is a competitive enzyme immunoassay for the quantitative analysis of Crystal Violet in tissue, feed and water.
The coupling antigens are coated on the micro-well stripes. The Crystal Violet in the  sample and the coupling antigens coated on the micro-well stripes compete for the anti-Crystal Violet antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration.
The optical density (OD) value of the sample has a negative correlation with the Malachite green in it. The measurement is made photometrically at 450 nm. The absorption is inversely proportional to the Crystal Violet concentration in the sample.
 

2. Technical specifications
Sensitivity : 0.05ppb     Detect time: Approx. 75 min
Detection limit:
Tissue ........................................0.10 ppb
Water.........................................0.50ppb
Feed............................................1.0 ppb
Recovery rate:
Tissue and feed......................75±10%    
Water.........................................80±20%
Cross-reaction rate:
Crystal Violet............................... 100% 
 

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ECALBIO CO.,LTD
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Add: HZAU, Luoshi Road, Wuhan City 430075, China. 
 

Ecalbio Co.,Ltd specialize in developing reagents of immunodiagnostic technology and biological systems with higher accuracy andreliability for results of the highest quality.

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ECALBIO CO.,LTD

HZAU, Luoshi road, Wuhan 430060, China
Tel:86-27-87745856
Email:info@ecalbio.com
Website: www.ecalbio.com