1. Principle
Malachite green ELISA kit is a competitive enzyme immunoassay for the quantitative analysis of Malachite green in tissue, feed and water.
The coupling antigens are coated on the micro-well stripes. The Malachite green in the sample and the coupling antigens coated on the micro-well stripes compete for the anti-Malachite green antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration.
The optical density (OD) value of the sample has a negative correlation with the Malachite green in it. The measurement is made photometrically at 450 nm. The absorption is inversely proportional to the Malachite green concentration in the sample.
2. Technical specifications
Sensitivity : 0.05 ppb Detect time: Approx. 75 min
Detection limit:
Tissue and water.........................................0.5 ppb
Feed............................................................1.0 ppb
Recovery rate:
Tissue and feed..........................................75 ± 10%
Water...........................................................80 ± 20%
Cross-reaction rate:
Malachite green..............................................100%
Leucomalachite Green ...................................100%
Crystal Violet ................................................. 95%
Leucocrystal Violet .........................................95%
ECALBIO CO.,LTD
Email: info@ecalbio.com Https: //www.ecalbio.com
Tel: +86-27-87745856 Mobi: +86-18963964485
Add: HZAU, Luoshi Road, Wuhan City 430075, China.
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Ecalbio Co.,Ltd specialize in developing reagents of immunodiagnostic technology and biological systems with higher accuracy andreliability for results of the highest quality.
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ECALBIO CO.,LTD
HZAU, Luoshi road, Wuhan 430060, China
Tel:86-27-87745856
Email:info@ecalbio.com
Website: www.ecalbio.com
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