1. Principle
Bacitracin ELISA kit is a competitive enzyme immunoassay for the quantitative analysis of bacitracin in the pork, beef and tissue,etc.
The coupling antigens are coated on the micro-well stripes. The Bacitracin  in the sample and the coupling antigens coated on the micro-well stripes compete for the anti-Bacitracin  antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration.
The optical density (OD) value of the sample has a negative correlation with the Bacitracin  in it. The measurement is made photometrically at 450 nm. The absorption is inversely proportional to the Bacitracin concentration in the sample.
 
2. Technical specifications
Sensitivity : 2.0ppb                Detect time: 45min
Detect Limit：
Tissue..........................................2 ppb
Cross-reaction rate:
Tissue..........................................100%
Recovery rate:
Tissue...........................................85 ± 20% 
 

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ECALBIO CO.,LTD
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Ecalbio Co.,Ltd specialize in developing reagents of immunodiagnostic technology and biological systems with higher accuracy andreliability for results of the highest quality.

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ECALBIO CO.,LTD

HZAU, Luoshi road, Wuhan 430060, China
Tel:86-27-87745856
Email:info@ecalbio.com
Website: www.ecalbio.com