Human Obestatin ELISA Kit

Cat NO. PRS-01916hu

FOR RESEARCH USE ONLY. Not FOR CLINICAL DIAGNOSIS USE.

Purpose

This kit allows for the determination of Obestatin concentrations in Human serum, blood plasma, and other biological fluids.

Principle of the assay

The kit assay Human Obestatin level in the sample，use Purified Human Obestatin antibody to coat microtiter plate wells, make solid-phase antibody, then add Obestatin to wells, Combined Obestatin antibody which With HRP labeled，become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Obestatin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Materials provided with the kit

Materials provided with the kit	48determinations	96 determinations	Storage
User manual	1	1
Closure plate membrane	2	2
Sealed bags	1	1
Microelisa stripplate	1	1	2-8℃
Standard：360 pg/ml	0.5ml×1 bottle	0.5ml×1 bottle	2-8℃
Standard diluent	1.5ml×1 bottle	1.5ml×1 bottle	2-8℃
HRP-Conjugate reagent	3ml×1 bottle	6ml×1 bottle	2-8℃
Sample diluent	3ml×1 bottle	6ml×1 bottle	2-8℃
Chromogen Solution A	3ml×1 bottle	6ml×1 bottle	2-8℃
Chromogen Solution B	3ml×1 bottle	6ml×1 bottle	2-8℃
Stop Solution	3ml×1 bottle	6ml×1 bottle	2-8℃
wash solution	（20ml×20 fold） ×1bottle	（20ml×30 fold） ×1bottle	2-8℃

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