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Ciprofloxacin (CPFX) ELISA Test Kit
Ciprofloxacin (CPFX) ELISA Test Kit
Origin of place China
Model
Supplier Nanjing Pars Biochem CO., LTD
Price info@pariselements.com
Hits 289
Updated 9/16/2021
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Ciprofloxacin (CPFX) ELISA Test Kit

 

Specification

96T/kit        96T*5/kit

 

Principle of Test

The Ciprofloxacin (CPFX) ELISA Test Kit is based on a competitive colorimetric ELISA assay.

The ciprofloxacin antibody has been coated in the plate wells. During the analysis, sample is added along with the ciprofloxacin-horseradish peroxidase (Ciprofloxacin-HRP) conjugate. If the ciprofloxacin residue is present in the sample, it will compete for the ciprofloxacin antibody, thereby preventing the Ciprofloxacin-HRP from binding to the antibody attached to the well, last, take coloration with TMB substrate. The samples A value is an inverse relationship with CPFX residue content. Lastly, compared with the standard curve can be concluded the CPFX residues in sample.

 

Composition of the Kit

Microelisa Stripplate, Standard , Enzyme conjugate, Antibody working solution , Concentrated Wash Solution 20×, concentrated Redissolving solution , Substrate A solution, Substrate B solution, Stop Solution.

 

Technique Data

1. Kit sensitivity: 0.1 ppb (ng/ml)

2.Reactive mode: 25℃, 45min~15min

3.Detection Limits:

Sample

Detection Limits

Tissue(chicken, pork, fish, shrimp)

0.3ppb

Honey

0.4ppb

Milk

3ppb

Milk powder

6ppb

Egg

3ppb

4.Cross-reaction rate:

Name

Cross-reaction rate

Ciprofloxacin

100%

Oxolin acid

28%

Levofloxacin

10%

Lomefloxacin

4%

Marbofloxacin

4%

Sarafloxacin

110%

5. Recovery rate:

Tissue, honey, eggs, milk, milk powder ……………85±15%

 

Storage Conditions

1.The kit shall be stored at [2-8 ℃]. Expiry date: 12 months

2.The opened Microelisa Stripplate can be stored at [2-8 ℃] and avoid damp. Use for at least 2 months.

months.

 

 

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