Optimised formats to suit your throughput requirements

CytoSure Genomic DNA Labelling Kits are available in 2 formats to suit your throughput requirements. The CytoSure Genomic DNA Labelling Kit enables labelling of 24 samples and is ideal for labs running one or two arrays a week. The CytoSure HT Genomic DNA Labelling Kit is designed specifically for high-throughput labs and allows simultaneous labelling of 96 samples. This plate-based format offers faster sample processing, a lower cost per sample and decreased sample-to-sample variation using a master mix format. It is also easier to automate and track samples.

Our lab has tried a variety of different labelling methods; and found OGT's CytoSure Genomic DNA Labelling Kit to give consistently low DLRS values and excellent signal-to-noise ratios". Cytogenetics Laboratory, Baylor College of Medicine

Reliable high quality results

CytoSure Genomic DNA Labelling Kits have been rigorously tested with a wide range of sample types to ensure optimal performance. The unique formulation ensures superior signal-to-noise ratios allowing confident detection of copy number variation (Figure 1). This high signal-to-noise ratio means that even small aberrations can be reliably detected (Figure 2).

 

Figure 1: High signal intensity allowing easy and accurate spot detection. Control DNA (Promega) was labelled using the CytoSure Genomic DNA Labelling Kit and another commercially available kit (Supplier A). The DNA labelled using the CytoSure Genomic DNA Labelling Kit exhibited far superior Cy3 signal intensity.

Figure 2: Reliable detection of small aberrations. DNA labelled using the CytoSure Genomic DNA Labelling Kit was run on a CytoSure ISCA 8 x 60k array. CytoSure Interpret Software combined with high DNA signal intensity allowed detection of a small (130kb) DNA amplification.

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Fast and simple procedure

CytoSure Genomic DNA Labelling Kits offer much faster DNA labelling and clean up than traditional enzymatic labelling procedures. Labelling reactions using both the 24 and 96 reaction kits can easily be completed in a single day (Figure 3). The procedure can also be automated for implementation in high-throughput workflows.

Figure 3: Two typical labelling workflows: With no need to digest, CytoSure Genomic DNA Labelling Kits save you at least 2 hours.

Everything you need, from reagents to plasticware

OGT offers the complete labelling solution — protocols, reagents, clean-up plates or columns and collection tubes. The unique kit formulation has been optimised for use with CytoSure arrays, which offer a wide variety of content and formats to suit your specific requirements. CytoSure arrays utilise 60-mer oligonucleotide probes, which are proven to offer higher signal-to-noise ratios and increased sensitivity and specificity¹.

CytoSure products provide a complete and integrated solution for cytogenetics research in your lab — from DNA labelling and arrays to analysis software and full workflow automation.

Oxford Gene Technology (OGT) provides world-class genetics research solutions to leading clinical and academic research institutions. Founded by Professor Sir Edwin Southern, and with customers in over 60 countries worldwide, OGT has a strong reputation and increasing share in the large and growing genomic medicine market. The Company’s Cytocell®, CytoSure™ and Genefficiency™ range of fluorescence in situ hybridisation (FISH), microarray and next generation sequencing (NGS) products and services deliver high-quality genetic analysis, enabling accurate identification and confirmation of the causative variation underlying genetic disease.