Principles
Ehrlichia Test Kit is designed to detect Ehrlichia canis antibodies in whole blood, serum or plasma. After being absorbed into the cellulose pad, the antibodies of Ehrlichia canis move and bind with gold-colloid complex of Ehrlichia canis antigen of the conjugate pad, forming Ag-Ab complex. This complex, then, forms Ag-Ab-Ag direct sandwich binding with p30/p30-1 antigen (Ag) of Ehrlichia canis in the nitrocellulose membrane. The test results can appearance on Control and Test lines where the principles of immunochromatography are used.
Contents
1
|
Test device
|
10 pcs
|
2
|
Dropper
|
10 pcs
|
3
|
Dilution Buffer
|
1 bottle
|
4
|
Instruction
|
1 insert
|
Procedure
1) Specimen Canine Whole blood, serum or plasma
2) Test procedure
a) When specimen and test kits are stored at cold circumstances ( 2~8 ℃), put them at room temperature for 15 ~ 30 minutes before use.
b) Take out a device from a pouch and place it on a horizontal surface.
c) Using a dropper as a pipette, obtain the specimen and dispense 10ul (black line) of the fluid into the specimen well (S).
d) When the specimen is completely absorbed into the specimen well (S), add 3 drops ( 80 ㎕ ) of buffer.
e) Read the test results between 5 ~ 10 minutes.
Results
1. Positive:Both on T Line and C line being seen wine-red color reaction.

2. Negative:no color reaction on Test Line (T Line),only on Control Line(C Line) being seen wine-red color reaction.

3. Void:No color reaction on C Line.

Package
10 tests/kit
Shelf Life
24 months (Lot No. printed on aluminum foil)
Storage
2-30 ℃
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