The kit is for the quantitative level of Leptin in the sample, adopt purified Bird Leptin to coat microtiter plate, make
solid-phase antibody, then add Leptin to wells, Combine Leptin antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color
at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured
at a wavelength of 450 nm. The concentration of Leptin in the samples is then determined by comparing the O.D. of
the samples to the standard curve.

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